Methods of determining seed vigor

1. Physical test method: Seed size, seed density, physical soundness etc.
2. Performance test in optimum condition: First count, Speed of germination, Seedling growth rate, Seedling length, Root:Shoot ratio, DM production, Vigor index length, Vigor index mass, Seed metabolic efficiency (SME), Mobilization efficiency (ME).
3. Stress test: Accelerated ageing test, Brick gravel test (Hiltner test), Paper piercing test, Compact soil test, Pathogen infested soil test, low or high pH test, Cold test, Exhaustion test, Water sensitivity test, Osmotic stress test, Oxygen stress test.
4. Biochemical test: Electrical conductivity test, Tetrazolium test, Respiration test, Glutamic acid decarbo-oxylase activity (GADA) test, Mitochondrial efficiency test, Volatile aldehyde test, Free fatty acid test.