About Lesson
Application of Autopolyploidy in Crop improvement
A) Triploids
- Triploids are produced by hybridization between tetraploid and diploid strains.
- They are generally highly sterile, except in a few cases.
- This feature is useful in the production of seedless watermelons.
- In certain species, they may be more vigorous than the normal diploids, e.g., in sugarbeets. These two examples are described in some detail.
- Seedless watermelons are produced by crossing tetraploid (4x, used as female) and diploid (2x, used as male) lines, since the reciprocal cross (2x x 4x) is not successful.
- The triploid plants do not produce true seeds; almost all the seeds are small, white rudimentary structures like cucumber (cucumis stivus) seeds. But few normal size seeds may occur which are generally empty. For good seed setting pollination is essential.
- For this purpose, diploid lines are planted in the ratio 1 diploid : 5 triploid plants.
- There are several problem viz. genetic instability of 4x lines, irregular fruit shape, a tendency towards hollowness of fruits, production of empty seeds and the labour involved in triploid seed production.
Limitations of auto polyploidy:
- Larger size autopolyploid generally contain more water and produce less dry matter content than diploids
- High sterility with poor seed setting is observed
- Due to complex segregation, progress through selection is slow
- Monoploids and triploids cannot be maintained except through clonal propagation
- The varieties cannot be produced at will
- Effects of auto polyploidy cannot be predicted.
Segregation in Auto tetraploids
- Depending upon the number of dominant alleles present, they are referred as simplex (Aaaa), duplex (AAaa), triplex (AAAa), Quadruplex (AAAA) and nulliplex (aaaa).
- On selfing a simplex will produce two types of gametes Aa and aa in 1:1 ratio due to random chromosome segregation.
- Self-pollination of such a simplex would produce. There genotypes AAaa, Aaaa and aaa in the ratio 1:2:1 giving the phenotypic ratio of 3 : 1. while produces 3 types of gametes viz., AA, Aa and aa in the ratio of 1:12:15 due to random chromatid segregation selfing of a simplex in such a case is expected produce the following progeny.
Note:
Quadraplix AAAA 1
Triplex AAA a 24
Duplex AAaa 174
Simplex Aaaa 360
Nulliplex aaaa 225
Total: 784