About Lesson
Restriction enzyme
- Enzyme produced by bacteria as defense mechanism against viral genome by cutting of the DNA of phase and hence inactivating it.
- They are also called as biological scissors.
Types of restriction enzyme
a) Type I: Recognize specific sequence; cuts everywhere.
b) Type II: Recognize specific sequence; cleavage.
c) Type III: Between type I and II.
Palindromes:
- Sequence recognized by restriction enzyme.
- Eg: 5’GACGTC3– restriction enzyme –>’3’CTGCAG5’