About Lesson
Agrobacterium tumefaciens mediated transformation:
- T-DNA is removed from bacterium
- Plasmid is cut using a restriction enzyme
- Foreign DNA cut with same restriction enzyme and inserted into plasmid.
- Formation of recombinant plasmid takes place
- Recombinant Ti plasmid reinserted into bacterium
- T-DNA carrying inserted gene incorporated into plant cell chromosome
- Plant cell is then cultured
- A transgenic plant expressing inserted gene is then generated.