Bioassay:

• Bioassay is a combination of two words as bio-life and assay-determination.
• It is indoor experiments to see the efficacy of any pesticides against target organisms.

Objectives:

• To determine virulence.
• Comparisons among isolates.
• To identify host range.
• To see the effect on host range, temperature, RH, Humidity, Stage of insect

Factors considers during bioassay

• Choice of bioassay materials.
• Stage of host.
• Inoculation methods.
• Mortality assessment techniques.
• Environment conditions.
• Most fungus induced mortality 2-4 wks so stop testing bioassay after 5-6 wks.