Some Key points to remember

• Cosmid vector contain a minimum of 250 hp of λ DNA; 45 kb insertion.
• Gene transformation; natural vector ( tumifaciens and A. rhizogenes).
• Cry gene: bacillus thuringenesis
• Father of tissue culture: G Haberlandt.
• PCR developed by Kary Mullis.
• Plastics are degraded by Alcaligenes eutophus ;
• Herbicides degraded by Pseudomonas putida
• Hybridoma: Antibody production cells from healthy donor to treat cancer cells.
• Sequence recognized by restriction enzyme is called palindromes.
• Plastids: Extra chromosomal circular DNA structures that can replicate inside host cells.
• T_i : Tumor inducing
• α-biodiversity: Diversity within each ecosystem
• β-biodiversity: diversity between two ecosystems
• γ-biodiversity: Sum total of α and β.