Biochemistry Essentials: Rahul’s Quick Guide for Master’s Prep
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Some key points to remember

  • Enzymes are proteins.
  • Autotrophic (organism that can make their own food eg: Plants) requires only inorganic nutrients.
  • Central theme: Energy
  • Hydrogen bond in liquid water have energy of only about 20 kg/mol.
  • Stable structures of amphipathic compound are called “micelles”.
  • Hydrophilic : Dissolves in water.
  • Hydrophobic: Doesn’t dissolves in water.
  • Force holding non-polar region of molecules together is hydrophobic interaction.
  • Movement of particles from region of higher concentration to lower concentration is
  • Titration is used to determine the amount of an acid in a given solution.
  • Sorensen introduced the concept of pH.
  • Karle Sheela is pioneer corer in field of biochemistry.
  • 1828, Wonder synthesized organic compound  from inorganic compound.
  • Dextrin: Intermediate product of the hydrolysis of starch and Glycogen.
  • Chiral carbon: 4 carbon atoms attached. Different atom attached to carbon atom.
  • Lipid acts as reserve food material of plasma component.
  • Fat stored in plants and animals are
  • Nucleotide: Containing sugar + nitrogen bases
  • Nucleoside: Don’t contain sugar.
  • Aromatic amino acid: Phenylalanine and Tryosine
  • Oxytocin and vasoprein are nano peptides.
  • Amino acid that remains in aqueous solution form in living beings is zwitter ions.
  • Prosthetic group: Non-amino acid group of protein ( Co-enzyme).
  • Substrate molecules binds on active site of enzyme whereas inhibitor molecules  bind on allosteric site  ( Other than active site).
  • Enzyme :

a) Protein: Apoenzymeà enzyme tightly bound to apoenzyme is called prosthetic group.

b) Non-protein: Co-enzyme

  • AUG : Initiation codon
  • UAA: termination codon
  • tRNA: smallest polymeric form of RNA.
  • Synthesis of amino acid occurs mostly in roots and leaves.
  • Site of AA synthesis : mitochondria
  • Central dogma of molecular biology : Unidirectional flow of information.
  • Activation of AA takes place in cytosol.
  • Sucrose synthesis occurs in cytosol
  • Non-sense codon: UAA, UGA and UAG.
  • Sucrose degradation is activated by the enzyme invertase.
  • Saturated fatty acid includes Aspartic acid , Butyric acid , Lauric acid, Myristic acid, Steric acid.
  • Unsaturated fatty acids includes Linolenic acid, α-linolenic acid, Verbonic acid, palmitic acid.
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